Isolation of Borrelia burgdorferi from the blood of seven patients with Lyme disease

See Also: WORMSER_INSISTS_LYME_A_KNEE_DISEASE.htm

Wormser knows what DNA primers to use to detect borrelia- They aren't OspA primers.

Whatever primers they use to detect borrelia in ticks, such as RNA and flagellin DNA, are the same primers that can be used in humans. This is never done, even though they know OspA will rarely be detected in it's initial form, if at all, in a mammalian host, because of selection pressures.

Wormser recently published that he now all of a sudden knows how to detect Lyme in the blood (May 2005) ??

THIS IS BULL-SHIT; Wormser and borrelia in the blood

Note that below, Wormser thinks diarrhea is life threatening. LOL. Debunking the "Ceftriaxone is poison" study by Lenny- the Catastrophizer -Sigal

Contradictions in PURPLE

Isolation of Borrelia burgdorferi from the blood of seven patients with Lyme disease.
Am J Med. 1990 Jan;88(1):21-6.
Nadelman RB, Pavia CS, Magnarelli LA, Wormser GP.

Department of Medicine, New York Medical College, Valhalia.

PURPOSE: Borrelia burgdorferi, the etiologic agent of Lyme disease, has rarely been successfully cultured from blood. We report on seven patients from Westchester County, New York, with B. burgdorferi bacteremia diagnosed between April 1987 and August 1987. PATIENTS AND METHODS: One hundred thirty-two attempts to isolate spirochetes were made on blood specimens obtained from 104 patients. Twenty-two of these specimens were obtained from nine patients who had recently been bitten by Ixodes ticks but who were asymptomatic. Heparinized blood or serum specimens (0.2 to 0.4 mL) were inoculated onto 6 mL of modified Barbour-Stoenner-Kelly medium. Lyme serology was performed by enzyme-linked immunosorbent polyvalent, IgM, and IgG assays, fluorescent immunoassay, and microhemagglutination. RESULTS: Four of the seven patients had erythema migrans, two had facial nerve palsy, and one had a flu-like syndrome without rash. These patients represented 21% (four of 19) of all patients with the characteristic skin lesion who had blood cultures for B. burgdorferi, and 40% (two of five) of all those with facial nerve palsy. Serologic testing was frequently nonreactive; two patients had no detectable antibody on multiple sera by five different assays. All patients improved with antibiotic treatment, and had negative subsequent blood cultures, but five of seven had persistent complaints after completion of therapy. CONCLUSION: Culturing blood for B. burgdorferi may be useful in confirming the diagnosis of Lyme disease in selected patients. Use of spirochete blood cultures may facilitate a better understanding of the pathogenesis and natural history of Lyme disease.

PMID: 2294761 [PubMed - indexed for MEDLINE]

Transfusion. 1990 May;30(4):298-301. Related Articles, Links

Survival of Borrelia burgdorferi in human blood stored under blood banking conditions.

Nadelman RB, Sherer C, Mack L, Pavia CS, Wormser GP.

Department of Medicine, New York Medical College, Valhalla.

Hematogenous dissemination of organisms occurs in many spirochetal diseases, including Lyme disease and syphilis. Although syphilis has been transmitted by transfusion, in the vast majority of cases, only fresh blood products were involved, in part because Treponema pallidum survives poorly when refrigerated in citrated blood. Because of the rising incidence of Lyme disease in certain areas, whether its causative agent, Borrelia burgdorferi, could survive under blood banking conditions was studied. Dilutions of stock cultures of two strains of B. burgdorferi were inoculated into samples of citrated red cells (RBCs). Viable spirochetes were recovered from RBCs inoculated with 10(6) organisms per mL, after refrigeration for as long as 6 weeks. It is concluded that B. burgdorferi may survive storage under blood banking conditions and that transfusion-related Lyme disease is theoretically possible.

PMID: 2349627 [PubMed - indexed for MEDLINE]

Br J Ophthalmol. 1990 Jun;74(6):325-7. Related Articles, Links

Ocular Lyme disease: case report and review of the literature.

Kauffmann DJ, Wormser GP.

New York Medical College, Valhalla 10595.

Lyme disease is an emerging new spirochaetal disease in which ocular complications may arise. We have seen a 45-year-old woman who developed unilateral endophthalmitis leading to blindness during the course of this disease. Ocular tissue showed the characteristic spirochete. A literature review shows that the commonest ocular manifestation of Lyme disease is a mild conjunctivitis, but other symptoms may include periorbital oedema, oculomotor palsies, uveitis, papilloedema, papillitis, interstitial keratitis, and others. Ophthalmologists treating patients from Lyme disease endemic areas need to be aware of the protean clinical manifestation of this disease.

PMID: 2198927 [PubMed - indexed for MEDLINE]

South Med J. 1991 Oct;84(10):1263-5. Related Articles, Links

Life-threatening complications of empiric ceftriaxone therapy for 'seronegative Lyme disease'.

Nadelman RB, Arlin Z, Wormser GP.

Department of Medicine, New York Medical College, Valhalla 10595.

Lyme disease, now the most common tick-borne illness in the United States, has recently received much media attention, due in part to its potentially serious sequelae in untreated patients. Because a rare patient with late illness may lack antibodies to the etiologic agent, Borrelia burgdorferi, physicians may be tempted to give empiric antibiotics for illnesses that may not be Lyme disease. We have described a patient who, despite negative laboratory evidence for late Lyme disease, was treated for 3 weeks with intravenous ceftriaxone and sustained serious complications, including granulocytopenia, fever, hepatitis, and Clostridium difficile-associated diarrhea. We caution physicians to weight carefully the risks of empiric treatment for ill-defined medical problems, and to recognize the hazards of even "safe" medications.

PMID: 1925730 [PubMed - indexed for MEDLINE]

N Y State J Med. 1992 Jan;92(1):5-8. Related Articles, Links

Incidence and prevalence of Lyme disease in a suburban Westchester County community.

Alpert B, Esin J, Sivak SL, Wormser GP.

Division of General Internal Medicine, Department of Medicine, Westchester County Medical Center, Valhalla, NY 10595.

This study was designed to determine the incidence and prevalence of Lyme disease in a section of Chappaqua, NY, a residential community in which Lyme disease is epidemic, and to identify risk factors for this disease. On the basis of clinical history and baseline serologic testing, the overall prevalence of Lyme disease for 114 persons entering the study was 8.8%. The incidence during the 5-month study period of May through September 1989 was 2.6%; all three incident cases had erythema migrans (EM). Hours outdoors per week in play or exercise correlated with the occurrence of Lyme disease.

PMID: 1574232 [PubMed - indexed for MEDLINE]